Test for growth-promoting properties

For controlled conditions in the manufacture of your products and for monitoring, culture media are used to detect microorganisms. To ensure that the culture media have the promised quality and reliability, a test for growth-promoting properties should be carried out. This includes inoculation with microorganisms, observation of growth and analysis.

Different sewing media are used depending on the individual problem.

• Growth-promoting properties of solid culture media:

The test results provide information on whether the solid culture medium has the necessary growth-promoting properties for the microorganisms being tested. This is important in areas such as microbiology, medical devices and the pharmaceutical industry, where the cultivation of microorganisms is required for specific purposes.

We ensure that the culture medium contains all the necessary nutrients required for the growth of the specific microorganisms. The composition may vary depending on the organisms to be detected. The solid culture medium is inoculated with the desired microorganisms. This is done by applying a microorganism solution to the surface of the medium. The inoculated solid culture medium is incubated under the optimal conditions, including temperature and humidity, to enable growth.

After the incubation period, the growth of the microorganisms is observed. This can be done by the appearance of colonies, changes in the color of the medium or other visible signs. The results are compared with controls. Control media without certain nutrients or with known growth-inhibiting properties can be used to evaluate the specific effects of the tested medium.

If necessary, further analytical methods can be used to quantify growth or to characterize certain properties of the microorganisms.

• Growth-promoting properties of liquid culture media:

Liquid culture media are aqueous solutions that contain nutrients to support the growth of microorganisms. This test determines and evaluates the ability of a liquid nutrient medium and, if necessary, makes adjustments to the nutrient compositions to support the growth of the corresponding microorganisms. In its liquid state, this culture medium provides a homogeneous environment for the cultivation of microorganisms. This is particularly useful for the propagation of bacteria or yeasts in laboratory experiments. Growth-promoting properties in liquid culture media are tested by inoculating the media with the desired microorganism and then observing the growth.

The selected microorganism is introduced into the liquid culture medium. This can be done by directly adding a bacterial or yeast culture. The inoculated culture media are incubated under suitable conditions, such as temperature and aeration, to ensure optimal growth conditions. The growth of the microorganism is observed during the incubation period. The results are compared with controls, e.g. with a standard culture medium, to determine whether the tested culture medium meets the specifications.

For this purpose, a low CFU number of suitable model organisms is used and the growth in the liquid media to be tested is optically compared with a suitable reference of the same volume. In addition, analytical methods such as microscopy, counting of colony forming units (CFU) or biochemical tests can be used to characterize the extent and nature of the growth more precisely.

Guidelines:

• Eur. 2.6.12
• USP <61>

We will be happy to assist you with your questions about testing for growth-promoting properties at
freigabe@gfps.de
.